This antibody detects PI3 Kinase p85 (PIK3R3) at C-term.
Aufreinigung
Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 324~353 amino acids from the C-terminal region of Human PI3KR3. Genename: PIK3R3
PIK3R3
Reaktivität: Human
WB
Wirt: Kaninchen
Monoclonal
unconjugated
Applikationshinweise
ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/10-1/50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
PI3KR3 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The protein is expressed at highest levels in brain and testis. Lower levels are detected in adipose tissue, kidney, heart, lung and skeletal muscle. The protein contains 2 SH2 domains.Synonyms: PI3K regulatory subunit gamma, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit gamma, Phosphatidylinositol 3-kinase regulatory subunit gamma, p55PIK