NEFH Antikörper

Produktnummer ABIN361350

Produktdetails anti-NEFH Antikörper

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reaktivität: Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEFH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Spezifität: Specific for the ~200k Neurofilament H protein. It recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.

Kreuzreaktivität: Huhn, Säugetier

Aufreinigung: Protein G purified culture supernatant

Immunogen: Purified bovine NF-H

Klon: NAP4

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:5,000 IF: 1:500 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: total IgG fraction + 10 mM Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Details zu NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Andere Bezeichnung: NEFH (NEFH Produkte)

Hintergrund: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (2) and Alzheimer's disease (3). Anti-Neurofilament H Western blot of rat cortex lysate showing specific immunolableing of the ~ 200k NF-H protein.

Molekulargewicht: '200 kDa

Gen-ID: 4744

UniProt: P12036