Rho-related GTP-binding protein Antikörper

Produktnummer ABIN361366

Produktdetails anti-Rho-related GTP-binding protein Antikörper

Target: Rho-related GTP-binding protein (RhO (pan))

Reaktivität: Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Rho-related GTP-binding protein Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Spezifität: Specific for the ~ 39k rhodopsin protein.

Kreuzreaktivität: Amphibien, Säugetier

Aufreinigung: Protein G purified culture supernatant

Immunogen: Purified native bovine rhodopsin

Klon: 1D4

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 IHC: 1:100 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Referenzen für anti-Rho-related GTP-binding protein Antikörper (ABIN361366)

Cho, Song, Shin, Kim: "Neonatal disease environment limits the efficacy of retinal transplantation in the LCA8 mouse model." in: BMC ophthalmology, Vol. 16, Issue 1, pp. 193, (2017) (PubMed).

Kim, Park, Lee, Shin, Nickas, Kim, Cho: "Yap is essential for retinal progenitor cell cycle progression and RPE cell fate acquisition in the developing mouse eye." in: Developmental biology, Vol. 419, Issue 2, pp. 336-347, (2017) (PubMed).

Details zu Rho-related GTP-binding protein

Target: Rho-related GTP-binding protein (RhO (pan))

Andere Bezeichnung: RHO (RhO (pan) Produkte)

Substanzklasse: Chemical

Hintergrund: Rhodopsin is a photoreceptor protein found in retinal rods. It is a complex formed by the binding of retinaldehyde, the oxidized form of retinol, to the protein opsin and undergoes a series of complex reactions in response to visible light resulting in the transmission of nerve impulses to the brain. Mutation of the rhodopsin gene is a major contributor to various retinopathies such as retinitis pigmentosa. The disease-causing protein generally aggregates with ubiquitin in inclusion bodies, disrupts the intermediate filament network and impairs the ability of the cell to degrade non-functioning proteins which leads to photoreceptor apoptosis (Berson et al., 1991). Other mutations on rhodopsin lead to X-linked congenital stationary night blindness, mainly due to constitutive activation, when the mutations occur around the chromophore binding pocket of rhodopsin (Dryja et al.,1993). Several other pathological states relating to rhodopsin have been discovered including poor post-Golgi trafficking, dysregulative activation, rod outer segment instability and arrestin binding. Anti-Rhodopsin Immunohistochemical staining of mouse retinal section showing specific immunolabeling of the rhodopsin protein in the rod spherules. Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.

Molekulargewicht: '39 kDa

Gen-ID: 509933

UniProt: P02699