Dieser Metabotropic Glutamate Receptor 1 Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), Immunohistochemistry (IHC)
Spezifität
Specific for the ~125k monomer and the ~250k mGluR1a dimer. Immunolabeling blocked by preadsorption of antibody with the peptide used to generate the antibody.
Kreuzreaktivität
Maus, Ratte (Rattus)
Aufreinigung
Antigen Affinity Purified
Immunogen
Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004, Francesconi et al., 2004, Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003). Anti-mGluR1a Western blot of 10 ug of HEK 293 cells expressing mGluR1a and mGluR5 showing the specific immunolabeling of the ~125k monomer and the ~250k dimer of mGluR1a. The mGluR1a antibody shows no reactivity toward mGluR5.