RPH3A Antikörper (pSer234)

Produktnummer ABIN361489

Produktdetails anti-RPH3A Antikörper

Target: RPH3A (Rabphilin 3A (RPH3A))

Bindungsspezifität: pSer234

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RPH3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Spezifität: Specific for the ~82k rabphilin protein phosphorylated at Ser234. The immunolabeling of the rabphilin band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling

Kreuzreaktivität: Ratte (Rattus)

Homologie: mouse

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 IHC: 1:400 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Referenzen für anti-RPH3A Antikörper (ABIN361489)

Stephens, Powell, Taraska, Harris: "Imaging the rapid yet transient accumulation of regulatory lipids, lipid kinases, and protein kinases during membrane fusion, at sites of exocytosis of MMP-9 in MCF-7 cells." in: Lipids in health and disease, Vol. 19, Issue 1, pp. 195, (2020) (PubMed).

Details zu RPH3A

Target: RPH3A (Rabphilin 3A (RPH3A))

Andere Bezeichnung: RPH3A (RPH3A Produkte)

Synonyme: 2900002P20Rik antikoerper, AU022689 antikoerper, AW108370 antikoerper, CG11556 antikoerper, Dmel\\CG11556 antikoerper, exophilin-1 antikoerper, fc61h10 antikoerper, rph3a antikoerper, wu:fc61h10 antikoerper, rabphilin 3A antikoerper, Rabphilin antikoerper, rabphilin-3a, putative antikoerper, rabphilin-3A antikoerper, rabphilin 3A S homeolog antikoerper, rabphilin 3A homolog (mouse), a antikoerper, RPH3A antikoerper, Rph3a antikoerper, Rph antikoerper, Smp_145120 antikoerper, Tsp_00335 antikoerper, rph3a.S antikoerper, rph3aa antikoerper

Hintergrund: Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994,Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease(Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995,Foletti and Scheller, 2001). Anti-Phospho-Ser234 Rabphilin 3A Western blot of rat brain lysate showing specific immunolabeling of the ~82k rabphilin 3A phosphorylated at Ser234.

Molekulargewicht: '82 kDa

Gen-ID: 171039

UniProt: P47709

Pathways: Synaptic Vesicle Exocytosis