KDELR Antikörper (AA 192-212)

Produktnummer ABIN361676

Produktdetails anti-KDELR Antikörper

Target: KDELR (KDEL Receptor (KDELR))

Bindungsspezifität: AA 192-212

Reaktivität: Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KDELR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Spezifität: Detects ~25 kDa.

Kreuzreaktivität: Huhn, Rind (Kuh), Hund, Drosophila melanogaster, Hamster, Human, Affe, Maus, Schwein, Kaninchen, Ratte, Schaf, Xenopus laevis

Aufreinigung: Protein G Purified

Immunogen: A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH

Klon: KR-10

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:1000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml was sufficient for detection of KDEL receptor in 20 μg monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Details zu KDELR

Target: KDELR (KDEL Receptor (KDELR))

Andere Bezeichnung: KDEL Receptor (KDELR Produkte)

Synonyme: BEST:CK00230 antikoerper, CG5183 antikoerper, CK00230 antikoerper, Dmel\\CG5183 antikoerper, ERD2 antikoerper, Erd2 antikoerper, dmErd2 antikoerper, l(2)k00311 antikoerper, KDEL receptor antikoerper, KdelR antikoerper

Hintergrund: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity, and all are located within the cis-Golgi and its intermediate compartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).

Gen-ID: 618184

NCBI Accession: NP_001069963

UniProt: P33946

Pathways: Maintenance of Protein Location