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CAMKII gamma Antikörper

CAMK2G Reaktivität: Ratte WB, IHC, ELISA, IP, IF, ICC Wirt: Maus Monoclonal 22B1 unconjugated
Produktnummer ABIN361699
  • Target Alle CAMKII gamma (CAMK2G) Antikörper anzeigen
    CAMKII gamma (CAMK2G) (Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G))
    Reaktivität
    • 70
    • 58
    • 46
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Ratte
    Wirt
    • 55
    • 29
    • 1
    Maus
    Klonalität
    • 50
    • 35
    Monoklonal
    Konjugat
    • 40
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CAMKII gamma Antikörper ist unkonjugiert
    Applikation
    • 56
    • 35
    • 30
    • 23
    • 19
    • 13
    • 10
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Spezifität
    Detects phosphorylated CaMKII from rat tissues. This antibody is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β).
    Kreuzreaktivität
    Human, Maus, Ratte
    Aufreinigung
    Protein G Purified
    Immunogen
    Synthetic peptide
    Klon
    22B1
    Isotyp
    IgG1
    Top Product
    Discover our top product CAMK2G Primärantikörper
  • Applikationshinweise
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    1 μg/ml was sufficient for detection of 0.2 μg CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    -20°C
  • Feng, Szabo, Dziak, Opas: "Cytoskeletal disassembly and cell rounding promotes adipogenesis from ES cells." in: Stem cell reviews, Vol. 6, Issue 1, pp. 74-85, (2010) (PubMed).

  • Target
    CAMKII gamma (CAMK2G) (Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G))
    Andere Bezeichnung
    CaMKII (CAMK2G Produkte)
    Synonyme
    CAMK antikoerper, CAMK-II antikoerper, CAMKG antikoerper, 5930429P18Rik antikoerper, Camkg antikoerper, calcium/calmodulin dependent protein kinase II gamma antikoerper, calcium/calmodulin-dependent protein kinase II gamma antikoerper, CAMK2G antikoerper, Camk2g antikoerper
    Hintergrund
    CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
    Gen-ID
    25400
    NCBI Accession
    NP_037052
    UniProt
    P11275
    Pathways
    WNT Signalweg, Interferon-gamma Pathway, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of long-term Neuronal Synaptic Plasticity
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