HSP70/HSC70 Antikörper

Produktnummer ABIN361706

Produktdetails anti-HSP70/HSC70 Antikörper

Target: HSP70/HSC70 (HSC70-4) (Heat Shock Protein Cognate 4 (HSC70-4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSP70/HSC70 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoelectron Microscopy (IEM), Immunocytochemistry (ICC), Immunofluorescence (IF)

Spezifität: Detects ~72 (HSP) and ~73 kDa (HSC).

Kreuzreaktivität: Beluga, C. elegans, Huhn, Rind (Kuh), Hund, Drosophila melanogaster, Fisch, Meerschweinchen, Hamster, Human, Affe, Maus, Schwein, Pflanzen, Kaninchen, Ratte, Schaf, Xenopus laevis

Aufreinigung: Protein G Purified

Immunogen: Recombinant HSP70/HSC70

Klon: N27F3-4

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:50)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN361705 was sufficient for detection of HSP70/HSC70 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-HSP70/HSC70 Antikörper (ABIN361706)

Wang, Kojima, Mobley, West: "Proteomic analysis of urinary extracellular vesicles reveal biomarkers for neurologic disease." in: EBioMedicine, Vol. 45, pp. 351-361, (2019) (PubMed).

Preusse-Prange, Modrow, Schwark, von Wurmb-Schwark: "Detection of constitutive and inducible HSP70 proteins in formalin fixed human brain tissue." in: Forensic science international, Vol. 235, pp. 62-7, (2014) (PubMed).

Morshed, Ma, Latif, Davies: "How one TSH receptor antibody induces thyrocyte proliferation while another induces apoptosis." in: Journal of autoimmunity, Vol. 47, pp. 17-24, (2013) (PubMed).

Sun, Prince, Manjarrez, Scroggins, Matts: "Characterization of the interaction of Aha1 with components of the Hsp90 chaperone machine and client proteins." in: Biochimica et biophysica acta, Vol. 1823, Issue 6, pp. 1092-101, (2012) (PubMed).

Modrow, Preusse-Prange, Meyer, Harder, Schwark, von Wurmb-Schwark: "Highly reliable quantification of proteins such as members of the HSP70 superfamily based on the grey scale index via immune detection stained bands on a Western blot." in: Forensic science international, Vol. 222, Issue 1-3, pp. 256-8, (2012) (PubMed).

Mutsvunguma, Moetlhoa, Edkins, Luke, Blatch, Knox: "Theiler's murine encephalomyelitis virus infection induces a redistribution of heat shock proteins 70 and 90 in BHK-21 cells, and is inhibited by novobiocin and geldanamycin." in: Cell stress & chaperones, Vol. 16, Issue 5, pp. 505-15, (2011) (PubMed).

Chen, Prior, Dargusch, Roberts, Riek, Eichmann, Chiruta, Akaishi, Abe, Maher, Schubert: "A novel neurotrophic drug for cognitive enhancement and Alzheimer's disease." in: PLoS ONE, Vol. 6, Issue 12, pp. e27865, (2011) (PubMed).

Olkku, Leskinen, Lammi, Hynynen, Mahonen: "Ultrasound-induced activation of Wnt signaling in human MG-63 osteoblastic cells." in: Bone, Vol. 47, Issue 2, pp. 320-30, (2010) (PubMed).

Details zu HSP70/HSC70

Target: HSP70/HSC70 (HSC70-4) (Heat Shock Protein Cognate 4 (HSC70-4))

Andere Bezeichnung: HSP70/HSC70 (HSC70-4 Produkte)

Synonyme: BAP74 antikoerper, CG4264 antikoerper, Dmel\\CG4264 antikoerper, E(csp)1545 antikoerper, E(nd)195 antikoerper, HSC-70 antikoerper, HSC4 antikoerper, HSC70 antikoerper, HSC70-4 antikoerper, Hsc-4 antikoerper, Hsc4 antikoerper, Hsc4p antikoerper, Hsc70 antikoerper, Hsp-c4 antikoerper, Hsp70 antikoerper, anon-WO0118547.237 antikoerper, bs17d06.y1 antikoerper, dhsc70 antikoerper, hsc4 antikoerper, hsc70 antikoerper, hsc70-4 antikoerper, hsp70 antikoerper, i190 antikoerper, l(3)03550 antikoerper, l(3)L3929 antikoerper, l(3)j7A4 antikoerper, scd antikoerper, Heat shock protein cognate 4 antikoerper, Hsc70-4 antikoerper

Hintergrund: HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at http://www.HSP70.com.

Gen-ID: 3303

NCBI Accession: NP_005336

UniProt: P0DMV8, P0DMV9