TLR4 Antikörper (AA 420-435)

Produktnummer ABIN361724

Produktdetails anti-TLR4 Antikörper

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Bindungsspezifität: AA 420-435

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TLR4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Detects ~75-80 kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).

Kreuzreaktivität: Human, Maus

Aufreinigung: Protein A Purified

Immunogen: Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4

Anwendungsinformationen

Applikationshinweise:

• WB (1:500)
• IHC (1:50)
• optimal dilutions for assays should be determined by the user.

Kommentare:

2 μg/ml of ABIN361724 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-TLR4 Antikörper (ABIN361724)

Vercellotti, Dalmasso, Schaid, Nguyen, Chen, Ericson, Abdulla, Killeen, Lindorfer, Taylor, Belcher: "Critical role of C5a in sickle cell disease." in: American journal of hematology, Vol. 94, Issue 3, pp. 327-337, (2019) (PubMed).

Nakano, Furube, Morita, Wanaka, Nakashima, Miyata: "Astrocytic TLR4 expression and LPS-induced nuclear translocation of STAT3 in the sensory circumventricular organs of adult mouse brain." in: Journal of neuroimmunology, Vol. 278, pp. 144-58, (2015) (PubMed).

Takano, Ohkusa, Otani, Min, Kadoyama, Minami, Sano, Matsuyama: "Lipid A-activated inducible nitric oxide synthase expression via nuclear factor-κB in mouse choroid plexus cells." in: Immunology letters, Vol. 167, Issue 2, pp. 57-62, (2015) (PubMed).

Details zu TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Andere Bezeichnung: TLR4 (TLR4 Produkte)

Synonyme: ARMD10 antikoerper, CD284 antikoerper, TLR-4 antikoerper, TOLL antikoerper, Lps antikoerper, Ly87 antikoerper, Ran/M1 antikoerper, Rasl2-8 antikoerper, toll like receptor 4 antikoerper, toll-like receptor 4 antikoerper, TLR4 antikoerper, Tlr4 antikoerper

Hintergrund: The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90 kDa (6).

Gen-ID: 7099

NCBI Accession: NP_612564

UniProt: O00206

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteine