FKBP5 Antikörper

Produktnummer ABIN361795

Produktdetails anti-FKBP5 Antikörper

Target: FKBP5 (FK506 Binding Protein 5 (FKBP5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FKBP5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Detects ~51 kDa.

Kreuzreaktivität: Hund, Hamster, Human, Maus, Kaninchen, Ratte

Aufreinigung: Protein G Purified

Immunogen: Synthetic peptide corresponding to the residues of human FKBP51

Klon: Hi51B

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:2000)
• ICC/IF (1:1000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:2000 dilution was sufficient for detection of FKBP51 in ~50 μg total protein using WB analysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-FKBP5 Antikörper (ABIN361795)

Mata-Greenwood, Jackson, Pearce, Zhang: "Endothelial glucocorticoid receptor promoter methylation according to dexamethasone sensitivity." in: Journal of molecular endocrinology, (2015) (PubMed).

Details zu FKBP5

Target: FKBP5 (FK506 Binding Protein 5 (FKBP5))

Andere Bezeichnung: FKBP51 (FKBP5 Produkte)

Synonyme: FKBP5 antikoerper, FKBP-5 antikoerper, AIG6 antikoerper, FKBP51 antikoerper, FKBP54 antikoerper, P54 antikoerper, PPIase antikoerper, Ptg-10 antikoerper, D17Ertd592e antikoerper, Dit1 antikoerper, si:zc263a23.8 antikoerper, wu:fc31g11 antikoerper, wu:fl87b03 antikoerper, zgc:64082 antikoerper, FK506 binding protein 5 antikoerper, FKBP5 antikoerper, fkbp5 antikoerper, Fkbp5 antikoerper

Hintergrund: HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).

Gen-ID: 2289

NCBI Accession: NP_001139247

UniProt: Q13451