PDIA3 Antikörper

Produktnummer ABIN361815

Produktdetails anti-PDIA3 Antikörper

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PDIA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Spezifität: Detects ~57 kDa.

Kreuzreaktivität: Rind (Kuh), Hund, Meerschweinchen, Hamster, Human, Affe, Maus, Schwein, Kaninchen, Ratte

Aufreinigung: Protein G Purified

Immunogen: Human recombinant ERp57 (Grp58)

Klon: Map-ERp57

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:2000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

0.5 μg/ml of ABIN361814 was sufficient for detection of ERp57 in 10 μg of heat shock Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-PDIA3 Antikörper (ABIN361815)

Ortiz-Sandoval, Hughes, Dacks, Simmen: "Interaction with the effector dynamin-related protein 1 (Drp1) is an ancient function of Rab32 subfamily proteins." in: Cellular logistics, Vol. 4, Issue 4, pp. e986399, (2015) (PubMed).

Lynes, Raturi, Shenkman, Ortiz Sandoval, Yap, Wu, Janowicz, Myhill, Benson, Campbell, Berthiaume, Lederkremer, Simmen: "Palmitoylation is the switch that assigns calnexin to quality control or ER Ca2+ signaling." in: Journal of cell science, Vol. 126, Issue Pt 17, pp. 3893-903, (2013) (PubMed).

Details zu PDIA3

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Andere Bezeichnung: ERp57 (PDIA3 Produkte)

Synonyme: ER60 antikoerper, ERp57 antikoerper, ERp60 antikoerper, ERp61 antikoerper, GRP57 antikoerper, GRP58 antikoerper, HsT17083 antikoerper, P58 antikoerper, PI-PLC antikoerper, grp-58 antikoerper, 58kDa antikoerper, Erp antikoerper, Grp58 antikoerper, PDI antikoerper, PDI-Q2 antikoerper, PLC[a] antikoerper, Plca antikoerper, 1 antikoerper, 25D3-membrane-associated antikoerper, sb:cb825 antikoerper, erp57 antikoerper, grp58 antikoerper, pdia3 antikoerper, protein disulfide isomerase family A member 3 antikoerper, protein disulfide-isomerase A3 antikoerper, protein disulfide isomerase associated 3 antikoerper, protein disulfide isomerase family A, member 3 antikoerper, protein disulfide isomerase family A member 3 S homeolog antikoerper, PDIA3 antikoerper, Tsp_10062 antikoerper, Pdia3 antikoerper, pdia3 antikoerper, pdia3.S antikoerper

Hintergrund: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).

Gen-ID: 2923

NCBI Accession: NP_005304

UniProt: P30101

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis