Calnexin Antikörper (C-Term)

Produktnummer ABIN361827

Produktdetails anti-Calnexin Antikörper

Target: Calnexin (CANX)

Bindungsspezifität: C-Term

Reaktivität: Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Calnexin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Detects the C-terminal domain of Calnexin ~90 kDa. Weak detection in Chicken, Drosophila, and Xenopus tissues

Kreuzreaktivität: Huhn, Rind (Kuh), Hund, Drosophila melanogaster, Meerschweinchen, Hamster, Human, Affe, Maus, Schwein, Wachtel, Kaninchen, Ratte, Schaf, Xenopus laevis

Aufreinigung: Peptide Affinity Purified

Immunogen: Dog calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN361826 was sufficient for detection of Calnexin in 10 μg of HeLa cell lysate by ECL immunoblot analysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-Calnexin Antikörper (ABIN361827)

Quintas, Pérez-Núñez, Sánchez, Nogal, Hentze, Castelló, Revilla: "Characterization of the African Swine Fever Virus Decapping Enzyme during Infection." in: Journal of virology, Vol. 91, Issue 24, (2017) (PubMed).

Hernández-Tiedra, Fabriàs, Dávila, Salanueva, Casas, Montes, Antón, García-Taboada, Salazar-Roa, Lorente, Nylandsted, Armstrong, López-Valero, McKee, Serrano-Puebla, García-López, González-Martínez et al.: "Dihydroceramide accumulation mediates cytotoxic autophagy of cancer cells via autolysosome destabilization. ..." in: Autophagy, Vol. 12, Issue 11, pp. 2213-2229, (2017) (PubMed).

Gordo-Gilart, Hierro, Andueza, Muñoz-Bartolo, López, Díaz, Jara, Álvarez: "Heterozygous ABCB4 mutations in children with cholestatic liver disease." in: Liver international : official journal of the International Association for the Study of the Liver, (2015) (PubMed).

Gordo-Gilart, Andueza, Hierro, Martínez-Fernández, DAgostino, Jara, Alvarez: "Functional analysis of ABCB4 mutations relates clinical outcomes of progressive familial intrahepatic cholestasis type 3 to the degree of MDR3 floppase activity." in: Gut, Vol. 64, Issue 1, pp. 147-55, (2014) (PubMed).

Williams, Tulke, Ilegems, Berggren, Broberger: "Expression of nucleobindin 1 (NUCB1) in pancreatic islets and other endocrine tissues." in: Cell and tissue research, Vol. 358, Issue 2, pp. 331-42, (2014) (PubMed).

Martín-Acebes, Merino-Ramos, Blázquez, Casas, Escribano-Romero, Sobrino, Saiz: "The composition of West Nile virus lipid envelope unveils a role of sphingolipid metabolism in flavivirus biogenesis." in: Journal of virology, Vol. 88, Issue 20, pp. 12041-54, (2014) (PubMed).

Details zu Calnexin

Target: Calnexin (CANX)

Andere Bezeichnung: Calnexin (CANX Produkte)

Synonyme: CNX antikoerper, IP90 antikoerper, P90 antikoerper, 1110069N15Rik antikoerper, AI988026 antikoerper, Cnx antikoerper, D11Ertd153e antikoerper, canx antikoerper, clgn antikoerper, clnx antikoerper, cnx antikoerper, PP90 antikoerper, zgc:63524 antikoerper, wu:fe06b12 antikoerper, CANX antikoerper, calnexin antikoerper, CalNeXin antikoerper, calnexin L homeolog antikoerper, CANX antikoerper, Canx antikoerper, cnx-1 antikoerper, canx.L antikoerper, canx antikoerper, LOC100283947 antikoerper

Hintergrund: Calnexin, an abundant ~90 kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50 kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

Gen-ID: 403908

NCBI Accession: NP_001003232

UniProt: P24643

Pathways: MAPK Signalweg, Thyroid Hormone Synthesis