P4HB Antikörper (AA 409-509)

Produktnummer ABIN361829

Produktdetails anti-P4HB Antikörper

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Bindungsspezifität: AA 409-509

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser P4HB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Detects ~58 kDa.

Kreuzreaktivität: Rind (Kuh), Hund, Meerschweinchen, Hamster, Human, Mollusca, Maus, Schwein, Ratte, Schaf, Xenopus laevis

Aufreinigung: Peptide Affinity Purified

Immunogen: Rat PDI synthetic peptide (409-509 aa C-terminal) conjugated to KLH

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN361828 was sufficient for detection of PDI in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-P4HB Antikörper (ABIN361829)

Lauranzano, Pozzi, Pasetto, Stucchi, Massignan, Paolella, Mombrini, Nardo, Lunetta, Corbo, Mora, Bendotti, Bonetto: "Peptidylprolyl isomerase A governs TARDBP function and assembly in heterogeneous nuclear ribonucleoprotein complexes." in: Brain : a journal of neurology, Vol. 138, Issue Pt 4, pp. 974-91, (2015) (PubMed).

Leung, Wang, Mak, Ng, Leung: "Differential proteomic responses in hepatopancreas and adductor muscles of the green-lipped mussel Perna viridis to stresses induced by cadmium and hydrogen peroxide." in: Aquatic toxicology (Amsterdam, Netherlands), Vol. 105, Issue 1-2, pp. 49-61, (2011) (PubMed).

Details zu P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Andere Bezeichnung: PDI (P4HB Produkte)

Synonyme: DSI antikoerper, ERBA2L antikoerper, GIT antikoerper, P4Hbeta antikoerper, PDI antikoerper, PDIA1 antikoerper, PHDB antikoerper, PO4DB antikoerper, PO4HB antikoerper, PROHB antikoerper, PDA2 antikoerper, PDIP antikoerper, PDIR antikoerper, ERp59 antikoerper, Pdia1 antikoerper, Thbp antikoerper, p55 antikoerper, XPDIp antikoerper, pdi antikoerper, 1810041F13Rik antikoerper, AI661267 antikoerper, Pdip antikoerper, Pdipl antikoerper, p58 antikoerper, prolyl 4-hydroxylase subunit beta antikoerper, protein disulfide isomerase family A member 2 antikoerper, prolyl 4-hydroxylase, beta polypeptide antikoerper, protein disulfide isomerase family A member 2 L homeolog antikoerper, protein disulfide isomerase associated 2 antikoerper, prolyl 4-hydroxylase subunit beta L homeolog antikoerper, protein disulfide-isomerase antikoerper, P4HB antikoerper, PDIA2 antikoerper, P4hb antikoerper, pdia2.L antikoerper, Pdia2 antikoerper, p4hb.L antikoerper, LOC8281065 antikoerper

Hintergrund: The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1). Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3). PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5). PDI is an abundant 55 kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol (1). PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus (6). It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor (7).

Gen-ID: 287164

NCBI Accession: NP_001099245

UniProt: P04785

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism