Calreticulin Antikörper

Produktnummer ABIN361835

Produktdetails anti-Calreticulin Antikörper

Target: Calreticulin (CALR)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Calreticulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Detects ~63 kDa.

Kreuzreaktivität: Huhn, Rind (Kuh), Hund, Meerschweinchen, Hamster, Human, Affe, Maus, Schwein, Kaninchen, Ratte, Schaf

Aufreinigung: Peptide Affinity Purified

Immunogen: Human calreticulin synthetic peptide with a cysteine residue added and the peptide conjugated to KLH

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:1000 dilution of ABIN361834 was sufficient for detection of Calreticulin in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-Calreticulin Antikörper (ABIN361835)

Schultheis, Smith, Kiosses, Kraynyak, Wong, Oh, Broderick: "Delineating the Cellular Mechanisms Associated with Skin Electroporation." in: Human gene therapy methods, Vol. 29, Issue 4, pp. 177-188, (2019) (PubMed).

Koide, Kondo, Odkhuu, Ulziisaikhan, Ukaji, Yokochi, Umezawa: "Inhibition of receptor activator of nuclear factor-κB ligand- or lipopolysaccharide-induced osteoclast formation by conophylline through downregulation of CREB." in: Immunology letters, Vol. 161, Issue 1, pp. 31-7, (2014) (PubMed).

Kobayashi, Hoshinaga, Miura, Tokuda, Shigeoka, Murai, Horio: "Ascorbic acid deficiency decreases hepatic cytochrome P-450, especially CYP2B1/2B2, and simultaneously induces heme oxygenase-1 gene expression in scurvy-prone ODS rats." in: Bioscience, biotechnology, and biochemistry, Vol. 78, Issue 6, pp. 1060-6, (2014) (PubMed).

Frenz, Graalmann, Detje, Döring, Grabski, Scheu, Kalinke et al.: "Independent of plasmacytoid dendritic cell (pDC) infection, pDC triggered by virus-infected cells mount enhanced type I IFN responses of different composition as opposed to pDC stimulated with free ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 193, Issue 5, pp. 2496-503, (2014) (PubMed).

Kluge, Mack, Iyer, Pujol, Heigele, Learn, Usmani, Sauter, Joas, Hotter, Bibollet-Ruche, Plenderleith, Peeters, Geyer, Sharp, Fackler, Hahn, Kirchhoff: "Nef proteins of epidemic HIV-1 group O strains antagonize human tetherin." in: Cell host & microbe, Vol. 16, Issue 5, pp. 639-50, (2014) (PubMed).

Tongu, Harashima, Yamada, Harada, Harada: "Immunogenic chemotherapy with cyclophosphamide and doxorubicin against established murine carcinoma." in: Cancer immunology, immunotherapy : CII, Vol. 59, Issue 5, pp. 769-77, (2010) (PubMed).

Details zu Calreticulin

Target: Calreticulin (CALR)

Andere Bezeichnung: Calreticulin (CALR Produkte)

Synonyme: CRT antikoerper, RO antikoerper, SSA antikoerper, cC1qR antikoerper, crc antikoerper, crt antikoerper, Calregulin antikoerper, CG9429 antikoerper, Clr antikoerper, Dmel\\CG9429 antikoerper, anon-EST:Liang-2.11 antikoerper, anon-EST:Liang-2.17 antikoerper, anon-EST:fe3D7 antikoerper, clone 2.11 antikoerper, clone 2.17 antikoerper, eth antikoerper, potp antikoerper, cc1qr antikoerper, ssa antikoerper, NV19007 antikoerper, CRP55 antikoerper, ERp60 antikoerper, HACBP antikoerper, CHOT1 antikoerper, CHT1 antikoerper, CT1 antikoerper, AA589632 antikoerper, CRTR antikoerper, Creat antikoerper, CCDS1 antikoerper, calrl2 antikoerper, fc66d10 antikoerper, wu:fc66d10 antikoerper, wu:fj50c03 antikoerper, zgc:66153 antikoerper, calreticulin antikoerper, calreticulin L homeolog antikoerper, Calreticulin antikoerper, putative calreticulin antikoerper, solute carrier family 6 member 8 antikoerper, solute carrier family 6 (neurotransmitter transporter, creatine), member 8 antikoerper, CALR antikoerper, calr.L antikoerper, Crt antikoerper, crt antikoerper, CRT antikoerper, Calr antikoerper, calr antikoerper, LMJF_31_2600 antikoerper, Crc antikoerper, Slc6a8 antikoerper, SLC6A8 antikoerper, LOC8269812 antikoerper

Hintergrund: Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER), but has also been detected in the nucleus and nuclear envelop. Like many other ER proteins, it has the conserved ER retention KDEL (Lys-Asp-Glu-Leu) sequence at its C-terminus (1-3). CRT's three domains include a 180 residue N-terminal domain, a proline-rich P-domain (residues 189-288) that binds Ca2+ with high affinity and shares homology with calnexin (CNX) and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity (1,3). Recent studies suggest that this soluble ER protein has a multifunctional role. It appears to be involved in calcium storage and regulation as well as having a molecular chaperone activity. It has been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may also play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells (4, 5), and it has also been shown to be induced in response to various types of cell stress including amino acid deprivation (6). Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and therefore it crucially participates in the coordination of many functions by the cell (4, 5). Studies also suggest its involvement in a few diseases such as systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis (1).

Gen-ID: 811

NCBI Accession: NP_004334

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response