Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Spezifität
The antibody detects endogenous level of PDK1 only when phosphorylated at serine 241.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer241 (A-N-S (p) -F-V) derived from Human PDK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
pdpk1 antikoerper, MGC82080 antikoerper, PDK1 antikoerper, PDPK2 antikoerper, PRO0461 antikoerper, Pdk1 antikoerper, zgc:153787 antikoerper, 3'-phosphoinositide-dependent protein kinase 1 antikoerper, 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 antikoerper, ATPDK1 antikoerper, AtPDK1 antikoerper, T32M21.110 antikoerper, T32M21_110 antikoerper, zgc:77318 antikoerper, 3-phosphoinositide dependent protein kinase 1 antikoerper, 3-phosphoinositide dependent protein kinase 1 L homeolog antikoerper, 3-phosphoinositide dependent protein kinase-1 antikoerper, 3-phosphoinositide dependent protein kinase 1a antikoerper, 3-phosphoinositide-dependent protein kinase 1 antikoerper, 3'-phosphoinositide-dependent protein kinase 1 antikoerper, 3-phosphoinositide dependent protein kinase 1b antikoerper, PDPK1 antikoerper, pdpk1.L antikoerper, pdpk1 antikoerper, Pdpk1 antikoerper, pdpk1a antikoerper, LOC100380750 antikoerper, pdk-1 antikoerper, PDK1 antikoerper, pdpk1b antikoerper
Hintergrund
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.