The antibody detects endogenous level of FAK only when phosphorylated at tyrosine 861.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pTyr861 (H-I-Y (p) -Q-P) derived from Human FAK. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Sheng, Song, Zheng, Zhou, Lu, Zhao, Zhang, Gong: "Abnormal cleavage of APP impairs its functions in cell adhesion and migration." in: Neuroscience letters, Vol. 450, Issue 3, pp. 327-31, (2009) (PubMed).
Zheng, Wei, Wang, Gong, Zhang: "Surface characterization and cytocompatibility of three chitosan/polycation composite membranes for guided bone regeneration." in: Journal of biomaterials applications, Vol. 24, Issue 3, pp. 209-29, (2009) (PubMed).
Wu, Zhu, Shi, Zhang, Ma, Yu: "Zoledronate inhibits the proliferation, adhesion and migration of vascular smooth muscle cells." in: European journal of pharmacology, Vol. 602, Issue 1, pp. 124-31, (2008) (PubMed).
Target
FAK (PTK2)
(PTK2 Protein tyrosine Kinase 2 (PTK2))
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.