The antibody detects endogenous level of PKCth only when phosphorylated at serine 695.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer695 (N-F-S (p) -F-M) derived from Human PKCth. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
PRKCT antikoerper, nPKC-theta antikoerper, A130035A12Rik antikoerper, AW494342 antikoerper, PKC-0 antikoerper, PKC-theta antikoerper, PKCtheta antikoerper, Pkcq antikoerper, protein kinase C theta antikoerper, protein kinase C eta antikoerper, protein kinase C, theta antikoerper, PRKCQ antikoerper, PRKCH antikoerper, Prkcq antikoerper
Hintergrund
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.