Histone H3.1 Antikörper (pSer10)

Produktnummer ABIN362203

Produktdetails anti-Histone H3.1 Antikörper

Target: Histone H3.1 (HIST1H3B)

Bindungsspezifität: pSer10

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H3.1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Spezifität: The antibody detects endogenous level of Histone H3.1 only when phosphorylated at serine 10.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer10 (R-K-S (p) -T-G) derived from Human Histone H3.1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-Histone H3.1 Antikörper (ABIN362203)

Jiang, Li, Yang, Hawke, Zheng, Xia, Aldape, Wei, Guo, Chen, Lu: "PKM2 regulates chromosome segregation and mitosis progression of tumor cells." in: Molecular cell, Vol. 53, Issue 1, pp. 75-87, (2014) (PubMed).

Liao, Dong, Wu, Yang, Wang, Guo, Chen, Xu, Wen: "Resolvin D1 attenuates inflammation in lipopolysaccharide-induced acute lung injury through a process involving the PPAR?/NF-?B pathway." in: Respiratory research, Vol. 13, pp. 110, (2013) (PubMed).

Sun, Chen, Wang, Li, Xue, Qu, Zhang, Mu, Lu: "The protective effect of alpha lipoic acid on Schwann cells exposed to constant or intermittent high glucose." in: Biochemical pharmacology, Vol. 84, Issue 7, pp. 961-73, (2012) (PubMed).

Yang, Xia, Hawke, Li, Liang, Xing, Aldape, Hunter, Alfred Yung, Lu: "PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis." in: Cell, Vol. 150, Issue 4, pp. 685-96, (2012) (PubMed).

Details zu Histone H3.1

Target: Histone H3.1 (HIST1H3B)

Andere Bezeichnung: Histone H3.1 (HIST1H3B Produkte)

Synonyme: H3 histone antikoerper, Hist1h2ai antikoerper, histone cluster 1, H2ai-like1 antikoerper, histone H3.1 antikoerper, Hht21p antikoerper, Hist1h2ail1 antikoerper, H3.1 antikoerper, HHT21 antikoerper

Hintergrund: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Molekulargewicht: 17 kDa

NCBI Accession: NP_003521

UniProt: P68431