Cofilin1/2 (CFL1/2) (pTyr88) Antikörper

Produktnummer ABIN362442

Produktdetails

Target: Cofilin1/2 (CFL1/2)

Bindungsspezifität: pTyr88

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC)

Spezifität: The antibody detects endogenous level of cofilin1/cofilin2 only when phosphorylated at tyrosine 88.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr88 (A-T-Y (p) -E-T) derived from Human coflin1, cofilin2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen

Li, Ke, Li, Liu, Zhu, Li: "DGCR6L, a novel PAK4 interaction protein, regulates PAK4-mediated migration of human gastric cancer cell via LIMK1." in: The international journal of biochemistry & cell biology, Vol. 42, Issue 1, pp. 70-9, (2009) (PubMed).

Antigendetails

Target: Cofilin1/2 (CFL1/2)

Andere Bezeichnung: cofilin1/cofilin2 (CFL1/2 Produkte)

Synonyme: CFL antikoerper, cofilin 1 antikoerper, CFL1 antikoerper

Hintergrund: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Molekulargewicht: 19 kDa

UniProt: Q9Y281

Pathways: Tube Formation, CXCR4-mediated Signaling Events