Dieser HDAC4/HDAC5/HDAC9 Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), Immunohistochemistry (IHC)
Spezifität
The antibody detects endogenous level of HDAC4/HDAC5/HDAC9 only when phosphorylated at serine 246/259/220.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer246, 259, 220 (T-A-S (p) -EP) derived from Human HDAC4, HDAC5, HDAC9. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general,they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs, classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions (for example HDAC-14.3.3 complexes are retained in the cytosol) and by the class to which they belong (class 1 HDACs are predominantly nuclear whilst class 2 HDACs shuttle between the nucleus and cytosol). HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents.