EGFR Antikörper (AA 1170-1174)

Produktnummer ABIN362866

Dieses Anti-EGFR-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von EGFR in WB. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für EGFR Antikörper (AA 1170-1174) (ABIN362866)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EGFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-EGFR Antikörper

Bindungsspezifität: AA 1170-1174

Spezifität: The antibody detects endogenous level of total EGFR protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Peptide sequence around AA 1170-1174 (P-D-Y-Q-Q) derived from Human EGFR. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-EGFR Antikörper (ABIN362866)

Gui, Han, Zhang, Liang, Wang, Xuan, Yu, Shang: "Dimerization of ZIP promotes its transcriptional repressive function and biological activity." in: The international journal of biochemistry & cell biology, Vol. 44, Issue 6, pp. 886-95, (2012) (PubMed).

Yang, Zheng, Xia, Ji, Chen, Guo, Lyssiotis, Aldape, Cantley, Lu: "ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect." in: Nature cell biology, Vol. 14, Issue 12, pp. 1295-304, (2012) (PubMed).

Details zu EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Andere Bezeichnung: EGFR

Hintergrund: Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.

Molekulargewicht: 175 kDa

NCBI Accession: NP_005219

UniProt: P00533

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine