Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Spezifität
The antibody detects endogenous level of total CDC2 protein.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen. Specificity/Sensitivity CDC2 (Ab-15) antibody detects endogenous levels of total CDC2 protein.
Immunogen
Peptide sequence around AA 13-17 (G-T-Y-G-V) derived from Human CDC2. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Immunofluorescence: 1:100-1:200
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Kubara, Kernéis-Golsteyn, Studény, Lanser, Meijer, Golsteyn: "Human cells enter mitosis with damaged DNA after treatment with pharmacological concentrations of genotoxic agents." in: The Biochemical journal, Vol. 446, Issue 3, pp. 373-81, (2012) (PubMed).
Yao, Li, Liu, Huang, Guo: "SUMOylation-regulated protein phosphorylation, evidence from quantitative phosphoproteomics analyses." in: The Journal of biological chemistry, Vol. 286, Issue 31, pp. 27342-9, (2011) (PubMed).
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.