The antibody detects endogenous level of total MARCKS protein.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Immunogen
Peptide sequence around AA 156-160 (R-F-S-F-K) derived from Human MARCKS. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
MARCKS
Reaktivität: Ratte
ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Western blotting: 1:500-1:1000 Immunofluorescence: 1:100-1:200
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Olianas, Dedoni, Onali: "Signaling pathways mediating phosphorylation and inactivation of glycogen synthase kinase-3? by the recombinant human ?-opioid receptor stably expressed in Chinese hamster ovary cells." in: Neuropharmacology, Vol. 60, Issue 7-8, pp. 1326-36, (2011) (PubMed).
Target
MARCKS
(Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
80K-L antikoerper, MACS antikoerper, PKCSL antikoerper, PRKCSL antikoerper, KINC antikoerper, Macs antikoerper, marcks antikoerper, MGC89127 antikoerper, MARCKS antikoerper, macs antikoerper, wu:fb55c06 antikoerper, wu:fb92d04 antikoerper, wu:fc19b04 antikoerper, wu:fc44a10 antikoerper, zgc:110235 antikoerper, fc34g03 antikoerper, fc38c06 antikoerper, wu:fc34g03 antikoerper, wu:fc38c06 antikoerper, zgc:109978 antikoerper, myristoylated alanine rich protein kinase C substrate antikoerper, myristoylated alanine-rich protein kinase C substrate antikoerper, methyl binding domain106 antikoerper, myristoylated alanine-rich protein kinase C substrate b antikoerper, myristoylated alanine-rich protein kinase C substrate S homeolog antikoerper, myristoylated alanine-rich protein kinase C substrate a antikoerper, MARCKS antikoerper, Marcks antikoerper, marcks antikoerper, mbd106 antikoerper, marcksb antikoerper, marcks.S antikoerper, marcksa antikoerper
Hintergrund
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.