GHRL Antikörper (AA 103-117)

Produktnummer ABIN364403

Produktdetails anti-GHRL Antikörper

Target: GHRL (Ghrelin and Obestatin Prepropeptide (GHRL))

Bindungsspezifität: AA 103-117

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GHRL Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human GHRL / Ghrelin. This antibody is expected to recognise all reported isoforms.

Homologie: Percent identity by BLAST analysis: Human, Gibbon, Baboon, Monkey, Elephant (100%) Gorilla, Mouse, Hamster (93%) Marmoset, Rat, Pig (87%) Horse (80%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-QDILWEEAKEAPADK from the C-terminus of human GHRL / Ghrelin (NP_057446.1, NP_001128413.1, NP_001128416.1, NP_001128417.1, NP_001128418.1). Percent identity by BLAST analysis: Human, Gibbon, Baboon, Monkey, Elephant (100%), Gorilla, Mouse, Hamster (93%), Marmoset, Rat, Pig (87%), Horse (80%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2 μg/mL), Peptide-ELISA (1:8000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GHRL

Target: GHRL (Ghrelin and Obestatin Prepropeptide (GHRL))

Andere Bezeichnung: GHRL / Ghrelin Preproprotein (GHRL Produkte)

Hintergrund: Name/Gene ID: GHRL

Synonyms: GHRL, Ghrelin, Growth hormone secretagogue, Motilin-related peptide, Obestatin, Appetite-regulating hormone, MTLRP, Protein M46

Gen-ID: 51738

NCBI Accession: NP_057446, NP_001128413, NP_001128416, NP_001128417, NP_001128418

UniProt: Q9UBU3