Adenosine A2a Receptor Antikörper (AA 373-391)

Produktnummer ABIN364458

Produktdetails anti-Adenosine A2a Receptor Antikörper

Target: Adenosine A2a Receptor (ADORA2A)

Bindungsspezifität: AA 373-391

Reaktivität: Human, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Adenosine A2a Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Marke: IHC-plus™

Spezifität: Detects adenosine receptor A2a. This antibody does not detect other AR subtypes. Species cross-reactivity: human. Not yet tested in other species.

Homologie: Percent identity by BLAST analysis: Dog (100%) Gorilla, Marmoset, Panda (89%) Human, Horse (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide: ESHGDMGLPDVELLSHELK, corresponding to amino acids 373-391 of Dog Adenosine Receptor A2a. Percent identity by BLAST analysis: Dog (100%), Gorilla, Marmoset, Panda (89%), Human, Horse (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (1:50), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50. Western Blot: 1:1000 detects an ~45kD protein from canine striatum representing A2aAR. Immunohistochemistry (paraffin-embedded sections): 1:100. Staining of A2aAR in human hippocampus yields a pattern consistent with plasma membrane staining.

Kommentare:

Target Species of Antibody: Dog

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 1 mg/mL BSA, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu Adenosine A2a Receptor

Target: Adenosine A2a Receptor (ADORA2A)

Andere Bezeichnung: ADORA2A/Adenosine A2A Receptor (ADORA2A Produkte)

Synonyme: ADORA2A antikoerper, adora2a.1 antikoerper, zgc:194773 antikoerper, A2aR antikoerper, ADORA2 antikoerper, RDC8 antikoerper, A2AAR antikoerper, AA2AR antikoerper, A2ar antikoerper, ADENO antikoerper, Adora2l1 antikoerper, adora2a.2 antikoerper, zgc:193802 antikoerper, zgc:193822 antikoerper, adenosine A2a receptor antikoerper, adenosine A2a receptor a antikoerper, adenosine A2a receptor L homeolog antikoerper, adenosine A2a receptor b antikoerper, ADORA2A antikoerper, Adora2a antikoerper, adora2aa antikoerper, adora2a.L antikoerper, adora2a antikoerper, adora2ab antikoerper

Hintergrund: Name/Gene ID: ADORA2A
Subfamily: Adenosine
Family: GPCR

Synonyms: ADORA2A, Adenosine receptor A2a, ADORA2, A2a receptor, Adenosine A2a receptor, Adenosine A2 receptor, Adenosine receptor subtype A2a, HA2aR, RDC8

Gen-ID: 135

UniProt: P29274

Pathways: Neurotrophin Signalübertragung, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints