Dieser Progesterone Receptor Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
Spezifität
This antibody is specific for the ~90k PR-A isoform and the ~120k PR-B isoform phosphorylated at Ser190. Immunolabeling is blocked by the Phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
Kreuzreaktivität (Details)
Species reactivity (tested):Human.
Produktmerkmale
Synonyms: PR, PGR, NR3C3, Nuclear Receptor Subfamily 3 Group C Member 3
Aufreinigung
Affinity Chromatography on Protein G.
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the Phospho-Ser190 ofHuman progesterone Receptor.
PGR
Reaktivität: Human
IHC, StM
Wirt: Maus
Monoclonal
PGR-2694
unconjugated
Applikationshinweise
Western Blot: 1/1000. Immunohistochemitry (Frozen Sections, unpublished observations): 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Lagerung
-20 °C
Informationen zur Lagerung
Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005). The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000, Lin et al., 2003). Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor (Clemm et al., 2000). Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.Synonyms: NR3C3, Nuclear Receptor Subfamily 3 Group C Member 3, PGR, PR