Collagen Type I Antikörper
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- Target Alle Collagen Type I (COL1) Antikörper anzeigen
- Collagen Type I (COL1) (Collagen, Type I (COL1))
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Reaktivität
- Human, Maus, Säugetier, Fisch, Amphibien, Avian
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Collagen Type I Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- Spezifität
- Specific for the ~180 kDa Collagen I Alpha-1 Propeptide in Human lung fibroblast extract.
- Kreuzreaktivität (Details)
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Species reactivity (expected):most (if not all) Species of Mammals, Birds, Amphibians and Fishes.
Species reactivity (tested):Human and Mouse. - Aufreinigung
- Affinity Chromatography.
- Immunogen
- Peptide from the Human Collagen I Alpha-1 propeptide sequence.
- Isotyp
- IgG
- Top Product
- Discover our top product COL1 Primärantikörper
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- Applikationshinweise
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Western Blot: 1/1000. Immunohistochemitry: 1/100. The antibody also works well for Immunohistochemistry on Paraformaldehyde-FixedSections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 °C in10 mM sodium citrate ( pH 6.0)/ 0.1 % Tween-20). Note that in Paraffin Sections of Formaldehyde-Fixed fibrotic mouse lung tissue, itrecognizes collagen I molecules that are still associated with the cells in which they weresynthesized.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- Liquid in PBS. Contains no preservative
- Konservierungsmittel
- Without preservative
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store the antibody undiluted (in aliquots) at-20 °C.
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Effects of chronically increased VEGF-A on the aging heart." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 32, Issue 3, pp. 1550-1565, (2018) (PubMed).
: "In Vitro Identification of New Transcriptomic and miRNomic Profiles Associated with Pulmonary Fibrosis Induced by High Doses Everolimus: Looking for New Pathogenetic Markers and Therapeutic Targets." in: International journal of molecular sciences, Vol. 19, Issue 4, (2018) (PubMed).
: "Hypoxia Inducible Factors Modify Collagen I Fibers in MDA-MB-231 Triple Negative Breast Cancer Xenografts." in: Neoplasia (New York, N.Y.), Vol. 20, Issue 2, pp. 131-139, (2018) (PubMed).
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Effects of chronically increased VEGF-A on the aging heart." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 32, Issue 3, pp. 1550-1565, (2018) (PubMed).
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- Target
- Collagen Type I (COL1) (Collagen, Type I (COL1))
- Andere Bezeichnung
- Collagen Type I (COL1 Produkte)
- Synonyme
- collagen type III alpha 1 chain antikoerper, COL3A1 antikoerper
- Hintergrund
- Collagen is the most abundant protein in mammals with type 1 collagen being the most abundant of the 29 known collagens. Type 1 collagen is integral in the strengthening and structural support of many tissues including skin, tendon, artery walls, fibrocartilage, bone and teeth. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procoallagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005).Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
- Gen-ID
- 1277
- NCBI Accession
- NP_000079
- UniProt
- P02452
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