GABRA3 Antikörper (N-Term)

Produktnummer ABIN372630

Produktdetails anti-GABRA3 Antikörper

Target: GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))

Bindungsspezifität: N-Term

Reaktivität: Human, Ratte, Hund, Rind (Kuh), Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GABRA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Specific for the ~51k Alpha-3 subunit of the GABAA receptor in Western blots. Labeling is absent in Alpha-3 subunit knockout animals.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Bovine, Canine, Zebrafish and non-Human Primates.
Species reactivity (tested):Rat and Mouse.

Aufreinigung: Affinity Chromatography.

Immunogen: Fusion protein from N-terminal region of the Alpha-3 subunit of rat GABAA Receptor.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu GABRA3

Target: GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))

Andere Bezeichnung: GABRA3 (GABRA3 Produkte)

Hintergrund: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six Alpha's, four Beta's and four Gamma's, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for Alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a Gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different Alpha-subunits of the receptor (McKernan et al., 2000, Mehta and Ticku, 1998, Ogris et al., 2004, Pöltl et al., 2003).Synonyms: GABA A Receptor subunit alpha-3, GABRA-3, Gamma-aminobutyric acid receptor subunit alpha-3

Gen-ID: 24947

NCBI Accession: NP_058765

UniProt: P20236