NMDA 1 Receptor Antikörper (Splice Variant C2)

Produktnummer ABIN372676

Produktdetails anti-NMDA 1 Receptor Antikörper

Target: NMDA 1 Receptor (NMDA R1) (NMDA Receptor 1 (NMDA R1))

Bindungsspezifität: Splice Variant C2

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NMDA 1 Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This antibody recognizes the ~120k NR1 subunit of the NMDA Receptor containing the C2 splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C2 insert.

Kreuzreaktivität (Details): Species reactivity (expected):Human.
Species reactivity (tested):Mouse and Rat.

Aufreinigung: Affinity Chromatography.

Immunogen: Peptide from the NR1 subunit, C2 splice variant insert of Rat NMDA Receptor.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 1/1000. Immunohistochemistry on Frozen Sections: 1/1000-1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 50 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.

Buffer: 5 mM Ammonium Bicarbonate.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Referenzen für anti-NMDA 1 Receptor Antikörper (ABIN372676)

Brady, Diaz, Iuso, Everett, Valenzuela, Caldwell: "Moderate prenatal alcohol exposure reduces plasticity and alters NMDA receptor subunit composition in the dentate gyrus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 3, pp. 1062-7, (2013) (PubMed).

Grosshans, Clayton, Coultrap, Browning: "LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1." in: Nature neuroscience, Vol. 5, Issue 1, pp. 27-33, (2001) (PubMed).

Details zu NMDA 1 Receptor

Target: NMDA 1 Receptor (NMDA R1) (NMDA Receptor 1 (NMDA R1))

Andere Bezeichnung: NMDA Receptor 1 (NMDA R1 Produkte)

Synonyme: CG2902 antikoerper, DMNMDAR1 antikoerper, DNMDAR-I antikoerper, DNmdar antikoerper, DmelNmdar1 antikoerper, Dmel\\CG2902 antikoerper, DrNR1 antikoerper, NMDA antikoerper, NMDA R1 antikoerper, NMDAR1 antikoerper, NMdar1 antikoerper, NR1 antikoerper, NRI antikoerper, Nmdar antikoerper, dNR1 antikoerper, dnmdar-I antikoerper, nmr antikoerper, NMDA receptor 1 antikoerper, Nmdar1 antikoerper

Hintergrund: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). There are a number of different splice variants of the NR1 subunit (Foldes et al., 1994, Zukin and Bennett, 1995). Differential splicing of three exons in the NR1 subunit generates up to eight NR1 subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.Synonyms: GRIN1, Glutamate [NMDA] receptor subunit zeta-1, NMDAR1

Gen-ID: 24408

NCBI Accession: NP_058706

UniProt: P35439