Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
Spezifität
This antibody recognizes the ~180k NR2B subunit of the NMDA receptor. No reactivity towards the NR2A and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
Kreuzreaktivität (Details)
Species reactivity (tested):Human, Mouse and Rat.
Aufreinigung
Affinity Chromatography.
Immunogen
Fusion protein from the C-terminus of the NR2B subunit of rat NMDA Receptor.
Western blot: 1/1000. Immunoprecipitation: 3 μL per 200 μg lysate. Immunohistochemistry: 1/1000-1/2000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore in 50 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
Buffer
5 mM Ammonium Bicarbonate.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store the antibody undiluted (in aliquots) at-20 °C.
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Kurup, Zhang, Xu, Venkitaramani, Haroutunian, Greengard, Nairn, Lombroso: "Abeta-mediated NMDA receptor endocytosis in Alzheimer's disease involves ubiquitination of the tyrosine phosphatase STEP61." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 17, pp. 5948-57, (2010) (PubMed).
Xu, Kurup, Zhang, Goebel-Goody, Wu, Hawasli, Baum, Bibb, Lombroso: "Extrasynaptic NMDA receptors couple preferentially to excitotoxicity via calpain-mediated cleavage of STEP." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 29, pp. 9330-43, (2009) (PubMed).
Davies, Goebel-Goody, Coultrap, Browning: "Long term synaptic depression that is associated with GluR1 dephosphorylation but not alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor internalization." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33138-46, (2008) (PubMed).
Chernova, Steinert, Guerin, Nicotera, Forsythe, Smith: "Neurite degeneration induced by heme deficiency mediated via inhibition of NMDA receptor-dependent extracellular signal-regulated kinase 1/2 activation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 32, pp. 8475-85, (2007) (PubMed).
The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be clonedand it can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Overexpression of the NR2B-subunit of the NMDA receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a, Clayton et al., 2002b). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999).Synonyms: GRIN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NMDA Receptor 2B