This antibody recognizes the ~68k to ~70k PAK protein phosphorylated at Thr402. The immunolabeling of PAK is completely eliminated by lambda-phosphatase treatment.
Kreuzreaktivität (Details)
Species reactivity (expected):Human, Bovine, Canine and Mouse. Species reactivity (tested):Rat.
Aufreinigung
Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr402 of Rat p21 activated kinase 2 (PAK-2).
In mammals, there are several identified isoforms of p21-activated protein kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003, Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).Synonyms: Alpha-PAK, Beta-PAK, Gamma-PAK, OPHN3, Oligophrenin-3, PAK 1, PAK 2, PAK 3, PAK alpha, PAK beta, PAK gamma, PAK-1, PAK-2, PAK-3, PAK65, Serine/threonine-protein kinase PAK 1, Serine/threonine-protein kinase PAK 2, Serine/threonine-protein kinase PAK 3, p21-activated kinase 1, p21-activated kinase 2, p21-activated kinase 3, p58, p65-PAK