SYT1 Antikörper (pThr202)

Produktnummer ABIN372724

Produktdetails anti-SYT1 Antikörper

Target: SYT1 (Synaptotagmin I (SYT1))

Bindungsspezifität: pThr202

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Huhn, Hund, Zebrafisch (Danio rerio), Non-Human Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SYT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Spezifität: This antibody recognizes the ~60k-62k synaptotagmin protein phosphorylated at Thr202 in Western Blots of Rat brain lysate. The immunolabeling is completely eliminated by lambda-phosphatase treatment.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse, non-Human Primate and Zebrafish.
Species reactivity (tested):Rat.

Aufreinigung: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr202 of Synaptotagmin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Dot blot: 1/1,000. Western blot: 1/1,000. Immunohistochemistry: 1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Andere Bezeichnung: Synaptotagmin-1 (SYT1 Produkte)

Hintergrund: Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001, Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin's ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999, Lee et al., 2004).Synonyms: SYT1, SYT2, SYT3, SYT4, SYT5

Gen-ID: 6857

NCBI Accession: NP_001129277

UniProt: P21579

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport