PRAM1 Antikörper (C-Term)

Produktnummer ABIN374107

Produktdetails anti-PRAM1 Antikörper

Target: PRAM1 (PML-RARA Regulated Adaptor Molecule 1 (PRAM1))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PRAM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: DFCDPLENQP LPLGR

Spezifität: This antibody detects PRAM1 at C-term.

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_115528.3 Genename: PRAM1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 8000. Western blot: 1-3 μg/mL (overnight incubation). Approx 90-100 kDa band seen in Jurkat celllysates. Please note that although the predicted MW of PRAM-1 is 70 kDa, Moog-Lutz et al. ,(J. Biol. Chem, Jun 2001, 276: 22375 - 22381) detected aPRAM-1 migrating with an apparentmolecular mass of 97 kDa and this is what we observed as well.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumi

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu PRAM1

Target: PRAM1 (PML-RARA Regulated Adaptor Molecule 1 (PRAM1))

Andere Bezeichnung: PRAM1 (PRAM1 Produkte)

Hintergrund: Synonyms: PML-RARA-regulated adapter molecule 1, PRAM, PRAM-1

Gen-ID: 84106

NCBI Accession: NP_115528

UniProt: Q96QH2

Pathways: Regulation of Leukocyte Mediated Immunity