PLK4 Antikörper (N-Term)

Produktnummer ABIN374175

Produktdetails anti-PLK4 Antikörper

Target: PLK4 (Polo-Like Kinase 4 (PLK4))

Bindungsspezifität: N-Term

Reaktivität: Human, Hund, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PLK4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: ATCIGEKIED FKVGN

Spezifität: This antibody detects SAK / STK18 at N-term.

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_055079.3 Genename: PLK4

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 95 kDa band observed in Human Placenta lysates(calculated MW of 105 kDa according to NP_055079.2). An additional faint band of 55 kDawas consistently observed, however this band was not blocked by the immunizing peptideand it is therefore a non-specific signal. We call for caution when used for other assaysthan Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu PLK4

Target: PLK4 (Polo-Like Kinase 4 (PLK4))

Andere Bezeichnung: PLK4 / SAK / STK18 (PLK4 Produkte)

Substanzklasse: Phage Protein

Hintergrund: Synonyms: PLK-4, Polo-like kinase 4, Serine/threonine-protein kinase 18, Serine/threonine-protein kinase PLK4, Serine/threonine-protein kinase Sak

Gen-ID: 10733

NCBI Accession: NP_055079

UniProt: O00444

Pathways: M Phase