DKK3 Antikörper (C-Term)
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- Target Alle DKK3 Antikörper anzeigen
- DKK3
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Bindungsspezifität
- C-Term
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Reaktivität
- Human
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser DKK3 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB)
- Sequenz
- C-AAAALLGGEE I
- Spezifität
- This antibody detects Dickkopf-3 at C-term.
- Aufreinigung
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- Immunogen
- Peptide from C Terminus of the protein sequence according to NP_037385.2, NP_056965.3, NP_001018067.1 Genename: DKK3
- Top Product
- Discover our top product DKK3 Primärantikörper
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- Applikationshinweise
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Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.03-0.1 μg/mL. Approx.70 kDa band observed in lysates of cell line HEK293(calculated MW of 38.0 kDa according to NP_037385.2, NP_056965.3 andNP_001018067.1). The observed molecular weight corresponds to earlier findings inliterature (Krupnik et al, Gene.1999 Oct 1,238(2): 301-13. , PMID: 10570958 ).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.5 mg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- Target
- DKK3
- Andere Bezeichnung
- Dickkopf-3 (DKK3) (DKK3 Produkte)
- Synonyme
- REIC antikoerper, RIG antikoerper, reic antikoerper, dkk-3 antikoerper, AW061014 antikoerper, C87148 antikoerper, Reic antikoerper, p29 antikoerper, dickkopf WNT signaling pathway inhibitor 3 antikoerper, dickkopf WNT signaling pathway inhibitor 3 S homeolog antikoerper, DKK3 antikoerper, dkk3 antikoerper, dkk3.S antikoerper, Dkk3 antikoerper
- Hintergrund
- DKK3, like DKK1, DKK2, and DKK4, possesses an N terminal signal peptide and 2 conserved cysteine rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin type proteases.Synonyms: Dickkopf-related protein 3, Dkk-3, REIC
- Gen-ID
- 27122
- NCBI Accession
- NP_037385
- UniProt
- Q9UBP4
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