Peptide ELISA: Limit dilution 1: 128000. Western blot: Preliminary experiments gave bands at approx 150 kDa and 22-20 kDa inHuman Brain (Hippocampus) lysates after 0.1 μg/mL antibody staining. Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Adrenal Gland showsmembranous and fibrous staniing in and around secretory cells of the zona fasciculata inthe cortex. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Axin 1 is a negative regulator of Wnt signaling and is also associated with cancer. Axin 1 contains RGS (Regulator of G protein signaling domain) and Axin_b-cat_bind (Axin beta-catenin binding domain).Synonyms: AXIN, AXIN1, Axis inhibition protein 1