KLF8 Antikörper (N-Term)

Produktnummer ABIN374228

Produktdetails anti-KLF8 Antikörper

Target: KLF8 (Kruppel-Like Factor 8 (KLF8))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KLF8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: VDMDKLINNL EVQ-C

Spezifität: This antibody detects KLF8 at N-term.

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_009181.2, NP_001152768.1 Genename: KLF8

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 50 kDa band observed in Human Kidney lysates (calculatedMW of 39.3 acording to NP_009181.2). In glioblastomas this protein was reported to run atappr.60 kDa (Ding et al . J Biol Chem.2005 Feb 25,280(8): 6802-15, PMID: 15557280).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu KLF8

Target: KLF8 (Kruppel-Like Factor 8 (KLF8))

Andere Bezeichnung: KLF8 (KLF8 Produkte)

Hintergrund: Kruppel-like factor proteins all contain a characteristic C-terminal DNA binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers. KLF8 is a transcriptional repressor and activator. It also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK). Ubiquitously expressed.Synonyms: BKLF3, Basic krueppel-like factor 3, KLF8, Krueppel-like factor 8, ZNF7, Zinc finger protein 741

Gen-ID: 11279

NCBI Accession: NP_009181

UniProt: O95600