KLF4 Antikörper (N-Term)

Produktnummer ABIN374229

Produktdetails anti-KLF4 Antikörper

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KLF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequenz: AVSDALLPSF ST-C

Spezifität: This antibody detects KLF4 at N-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, CowSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_004226.2 Genename: KLF4

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 64000. Western blot: 0.1-0.3 μg/mL. Experiments gave strong bands at 75-90 kDa and a faint50- kDa band in Human Duodenal lysate after 0.1 μg/mL antibody staining. These bandscorrespond to earlier findings in literature (Piccinni et al, J. Biol. Chem. , Vol.279 (10),9103-9114). All these bands were successfully blocked by incubation with the immunizingpeptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Andere Bezeichnung: KLF4 (KLF4 Produkte)

Synonyme: EZF antikoerper, GKLF antikoerper, Gklf antikoerper, Zie antikoerper, KLF4 antikoerper, biklf antikoerper, biklf-A antikoerper, Kruppel like factor 4 antikoerper, Kruppel-like factor 4 (gut) antikoerper, Kruppel-like factor 4 antikoerper, Kruppel-like factor 4 (gut) L homeolog antikoerper, KLF4 antikoerper, Klf4 antikoerper, klf4 antikoerper, klf4.L antikoerper

Hintergrund: KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor.Synonyms: EZF, Epithelial zinc finger protein EZF, GKL, Gut-enriched krueppel-like factor, Krueppel-like factor 4

Gen-ID: 9314

NCBI Accession: NP_004226

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance