DCTN1
Reaktivität: Human, Maus, Ratte
IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Peptide ELISA: Limit dilution 1: 128000. Western Blot: 0.5-2 μg/mL. pprox 150 kDa band observed in cell lline A549 and HumanTestis lysates (calculated MW of 140 kDa according to NP_004073.2). Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Cerebellum showspixulate staining of cytoplasm in neuronal cells. In paraffin embedded Human Testisshows cytoplasm staining in cells of the seminiferous tubules. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
The DCTN1 gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein. Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy.Synonyms: 150 kDa dynein-associated polypeptide, DAP-150, DCTN1, DP-150, p135, p150-glued