NEIL1 Antikörper (C-Term)

Produktnummer ABIN374304

Produktdetails anti-NEIL1 Antikörper

Target: NEIL1 (Endonuclease VIII-Like 1 (NEIL1))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NEIL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequenz: C-DIPSLEPEGT SAS

Spezifität: This antibody detects NEIL1 at C-term.

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_078884.2 Genename: NEIL1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 16000. Western blot: Experiments gave an approx 60 kDa band in Human Liver and Brain lysates at1 μg/mL. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Placenta shows strongstaining of cytoplasm in syncytiotrophoblasts.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu NEIL1

Target: NEIL1 (Endonuclease VIII-Like 1 (NEIL1))

Andere Bezeichnung: NEIL1 (NEIL1 Produkte)

Hintergrund: Human NEIL1 is the homologs of Escherichia coli Nei. Human NEIL1 is a DNA glycosylase, that phylogenetically belongs to the Fpg/Nei family, primarily found in the bacterial kingdom and is functionally homologous to bacterial Nei. Thymine glycol, a potentially lethal DNA lesion produced by reactive oxygen species, can be removed by DNA glycosylases. The NEIL1 gene is located on the long arm of chromosome 15 that is frequently deleted in human cancers.Synonyms: DNA glycosylase/AP lyase Neil1, DNA-(apurinic or apyrimidinic site) lyase Neil1, Endonuclease VIII-like 1, FPG1, NEH1

Gen-ID: 79661

NCBI Accession: NP_078884

UniProt: Q96FI4

Pathways: DNA Reparatur