CARD11 Antikörper (C-Term)

Produktnummer ABIN374314

Produktdetails anti-CARD11 Antikörper

Target: CARD11 (Caspase Recruitment Domain Family, Member 11 (CARD11))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CARD11 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: C-QRKTIWVDED QL

Spezifität: This antibody detects CARD11 / CARMA1 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Dog, CowSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_115791.3 Genename: CARD11

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western Blot: 1-3 μg/mL. Approx 150+65 kDa bands observed in lysates of cell line Jurkat(calculated MW of 135 kDa according to NP_115791.2). A similar pattern was observed inHEK293 transiently expressing CARD11. These bands are not observed in thenon-transfected HEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu CARD11

Target: CARD11 (Caspase Recruitment Domain Family, Member 11 (CARD11))

Andere Bezeichnung: CARD11 / CARMA1 (CARD11 Produkte)

Hintergrund: CARD11 belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.Synonyms: CARD-containing MAGUK protein 3, Caspase recruitment domain-containing protein 11

Gen-ID: 84433

NCBI Accession: NP_115791

UniProt: Q9BXL7

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, BCR Signaling