NUP50 Antikörper (C-Term)

Produktnummer ABIN374317

Produktdetails anti-NUP50 Antikörper

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NUP50 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequenz: C-ELHKILLEKK DA

Spezifität: This antibody detects NUP50 at C-term. It is expected to recognise both human isoforms of this protein.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Cow.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_009103.2, NP_705931.1. Genename: NUP50

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.2-1 μg/mL. Approx 60 kDa band seen in Jurkat lysate (predicted MW 54 kDaaccording to NP_009103).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu NUP50

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Andere Bezeichnung: NUP50 (NUP50 Produkte)

Hintergrund: The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. NUP50 may serve as a binding site on the nuclear side of the pore complex for export receptor-cargo complexes. It interacts with multiple transport receptor proteins including p27Kip1. This interaction is required for correct intracellular transport and degradation of p27Kip1.Synonyms: NPAP60L, Nuclear pore-associated protein 60 kDa-like, Nucleoporin 50 kDa

Gen-ID: 10762

NCBI Accession: NP_009103

UniProt: Q9UKX7

Pathways: Tube Formation