This antibody detects NOXO1 at C-term. It is expected to recognise all three reported isoforms (as represented by NP_653204.1, NP_751907.1and NP_751908.1).
Kreuzreaktivität (Details)
Species reactivity (tested):Human
Aufreinigung
Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Immunogen
Peptide from the C Terminus of the protein sequence according to NP_653204.1, NP_751907.1, NP_751907.1. Genename: NOXO1
NOXO1
Reaktivität: Human
Wirt: Kaninchen
Polyclonal
FITC
Applikationshinweise
Peptide ELISA: Limit dilution 1: 16000. Immunohistochemistry: 10-20 μg/mL. In paraffin embedded Human Kidney Cortex showscytoplasmic stain in epithelial cells of some PCT. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
NOXO1 (Nox organizing protein 1) and NOXA1 (Nox Activating protein 1) are homologs of p47phox and p67phox. p47phox functions in phagocytes as an essential organizing protein mediating the binding of other regulatory proteins during activation of the phagocyte oxidase, and its translocation to the membrane is triggered upon cell activation by hyperphosphorylation, which relieves autoinhibition of SH3 and PX domains. NOXO1 lacks an auto inhibitory region and phosphorylation sites that are present in p47phox. Cotransfection of Nox1, NOXO1 and NOXA1 reconstitutes ROS (reactive oxygen species) generation in HEK 293 cells in the absence of cell stimulation. NOXO1 binds to the phosphatidylinositol (PtdIns) lipids PtdIns 3,5 P2, PtdIns 5 P and PtdIns 4 P. Unlike p47phox, which is located in the cytosol of resting cells and translocates to the plasma membrane where gp91phox is located, NOXO1 colocalizes with Nox1 in the membranes of resting cells. This localization of NOXO1 is dictated by its PX domain, since this domain but not the remainder of the molecule localizes to membranes.Synonyms: NADPH oxidase organizer 1, P41NOX, SH3PXD5