NIR1 Antikörper (C-Term)

Produktnummer ABIN374424

Produktdetails anti-NIR1 Antikörper

Target: NIR1 (PITPNM3) (PITPNM Family Member 3 (PITPNM3))

Bindungsspezifität: C-Term

Reaktivität: Human, Hund, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NIR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequenz: C-SWARGPPKFE SVP

Spezifität: This antibody detects NIR1 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, DogSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_112497.2, NP_001159438.1. Genename: PITPNM3

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 16000. Western blot: Preliminary experiments gave an approx 50 kDa band in Human Heart lysatesafter 0.1 μg/mL antibody staining. The 50 kDa band was successfully blocked by incubationwith the immunizing peptide. Immunohistochemistry: 5-10 μg/mL. In paraffin embeddedHuman Cerebral Cortex shows strong staining of cytoplasm in several neuronal cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu NIR1

Target: NIR1 (PITPNM3) (PITPNM Family Member 3 (PITPNM3))

Andere Bezeichnung: PITPNM3 (PITPNM3 Produkte)

Hintergrund: This gene encodes a member of a family of membrane-associated phosphatidylinositol transfer domain-containing proteins. The calcium-binding protein has phosphatidylinositol (PI) transfer activity and interacts with the protein tyrosine kinase PTK2B (also known as PYK2). The protein is homologous to a Drosophila protein that is implicated in the visual transduction pathway in flies. Mutations in this gene result in autosomal dominant cone dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene.Synonyms: Membrane-associated phosphatidylinositol transfer protein 3, NIR-1, NIR1, PITPNM 3, Phosphatidylinositol transfer protein, Pyk2 N-terminal domain-interacting receptor 1, membrane-associated 3

Gen-ID: 83394

NCBI Accession: NP_112497

UniProt: Q9BZ71