ACTR1A Antikörper (C-Term)

Produktnummer ABIN374428

Produktdetails anti-ACTR1A Antikörper

Target: ACTR1A (Alpha Centractin (ACTR1A))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ACTR1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: C-YEEDGARSIH RKT

Spezifität: This antibody detects Centractin alpha at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, PigSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_005727.1 Genename: ACTR1A

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 1000. Western blot: 1-3 μg/mL. Approx.45 kDa band observed in Human Kidney lysates(calculated MW of 42.5 kDa according to NP_005727.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu ACTR1A

Target: ACTR1A (Alpha Centractin (ACTR1A))

Andere Bezeichnung: Centractin alpha (ACTR1A Produkte)

Hintergrund: The actin-related protein ACTR1A, a member of the superfamily of actin-related proteins, is the major subunit of the dynactin complex, a key component of the cytoplasmic dynein motor machinery. The major components of the dynactin complex, consisting of at least ten polypeptides, include ACTR1A. ACTR1A, the most abundant subunit in the dynactin complex, is most similar to conventional actin (>50 % amino acid identity). The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dynein mediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1A overexpression in cells results in aberrant spindle morphologies and cell cycle delay at prometaphase, suggesting a possible function of ACTR1A/dynactin complex in progression through the prometaphase of mitosis.Synonyms: ACTR1A, ARP1, Actin-RPV, CTRN1, Centrosome-associated actin homolog

Gen-ID: 10121

NCBI Accession: NP_005727

UniProt: P61163

Pathways: M Phase