TPI1 Antikörper (C-Term)

Produktnummer ABIN374519

Produktdetails anti-TPI1 Antikörper

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TPI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody is expected to recognise both reported isoforms of Triosephosphate isomerase (TPI1).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine and Bovine.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-LKPEFVDIINAKQ, from the C Terminus of the protein sequence according to NP_000356.1, NP_001152759.1. Genename: TPI1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 0.01-0.03 μg/mL. Approx 26 kDa band observed in human liver and humanhepatoblastoma HepG2 cell lysates (calculated MW of 26.6 kDa according to NP_000356).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu TPI1

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Andere Bezeichnung: Triosephosphate Isomerase (TPI1) (TPI1 Produkte)

Hintergrund: Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.Synonyms: TIM, TPI, Triose-phosphate isomerase

Gen-ID: 7167, 9606

UniProt: P60174

Pathways: Cell RedoxHomeostasis