HNF1B Antikörper (Internal Region)

Produktnummer ABIN375249

Produktdetails anti-HNF1B Antikörper

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio), Hund, Rind (Kuh), Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser HNF1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Sequenz: C-QAYDRQKNPS KEER

Spezifität: This antibody is expected to recognize both reported isoforms (NP_000449.1 and NP_001159395.1).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine, Pig, Cow, Zebrafish.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein according to NP_000449.1, NP_001159395.1. Genename: HNF1B

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/64000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu HNF1B

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Andere Bezeichnung: HNF1 beta / TCF2 (HNF1B Produkte)

Hintergrund: TCF2 encodes transcription factor 2, a liver specific factor of the homeobox containing basic helix turn helix family. The TCF2 protein is believed to form heterodimers with another liver specific member of this transcription factor family, TCF1, depending on the TCF2 isoform, the result may be to activate or inhibit transcription of target genes. A mutation of TCF2 that disrupts normal function has been identified as the cause of MODY5 (Maturity Onset of Diabetes, Type 5). A third human transcript variant is believed to exist based on such a variant in the rat. However, to date such an mRNA species has not been isolated. TCF2 probably binds to the inverted palindrome 5' GTTAATNATTAAC 3'.Synonyms: HNF-1-beta, HNF-1B, Hepatocyte nuclear factor 1-beta, LFB3, TCF-2, Transcription factor 2, Variant hepatic nuclear factor 1, vHNF1

Gen-ID: 6928

NCBI Accession: NP_000449

UniProt: P35680

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation