UBE2I Antikörper (N-Term)

Produktnummer ABIN387910

Produktdetails anti-UBE2I Antikörper

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Bindungsspezifität: AA 1-30, N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UBE2I Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Homologie: Zf, C, M, Rat, X

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This UBC9 (UBE2I) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBC9 (UBE2I).

Klon: RB2811

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-UBE2I Antikörper (ABIN387910)

Lee, Kraus, Prins, Groenendyk, Aubry, Liu, Li, Julien, Touret, Sykes, Tremblay, Michalak: "UBC9-dependent association between calnexin and protein tyrosine phosphatase 1B (PTP1B) at the endoplasmic reticulum." in: The Journal of biological chemistry, Vol. 290, Issue 9, pp. 5725-38, (2015) (PubMed).

Fu, Xiong, Xu, Cheng, Tang: "MDA5 is SUMOylated by PIAS2? in the upregulation of type I interferon signaling." in: Molecular immunology, Vol. 48, Issue 4, pp. 415-22, (2011) (PubMed).

Details zu UBE2I

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Andere Bezeichnung: UBC9 (UBE2I) (UBE2I Produkte)

Hintergrund: UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, I?B?, and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.

Molekulargewicht: 18007

Gen-ID: 7329

NCBI Accession: NP_003336, NP_919235, NP_919236, NP_919237

UniProt: P63279

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway