Pan SUMO Antikörper

Produktnummer ABIN388089

Produktdetails

Target: Pan SUMO

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: "This Pan SUMO antibody recognizes SUMO2 and SUMO3. This antibody is generated from rabbits immunized with a recombinant protein encoding full length human SUMO3."

Klon: RB04602

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:10~50. WB: 1:1000. IHC-P: 1:100. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen

Dunphy, Luo, McBride: "Ehrlichia chaffeensis exploits host SUMOylation pathways to mediate effector-host interactions and promote intracellular survival." in: Infection and immunity, Vol. 82, Issue 10, pp. 4154-68, (2014) (PubMed).

Sun, Hunter: "Poly-small ubiquitin-like modifier (PolySUMO)-binding proteins identified through a string search." in: The Journal of biological chemistry, Vol. 287, Issue 50, pp. 42071-83, (2012) (PubMed).

Lutz, Wolters-Eisfeld, Joshi, Djogo, Jakovcevski, Schachner, Kleene: "Generation and nuclear translocation of sumoylated transmembrane fragment of cell adhesion molecule L1." in: The Journal of biological chemistry, Vol. 287, Issue 21, pp. 17161-75, (2012) (PubMed).

Antigendetails

Target: Pan SUMO

Hintergrund: Covalent modification of target lysines by SUMO (small ubiquitin-like modifier) modulates processes such as protein localization, transcription, nuclear transport, mitosis, DNA replication and repair, signal transduction, and viral reproduction. SUMO does not seem to be involved in protein degradation and may in fact function as an antagonist of ubiquitin in the degradation process. The SUMO family consists of SUMO1 and closely related homologs SUMO2, SUMO3, and SUMO4. Sumoylation has been shown to regulate a wide range of proteins, including MDM2, PIAS, PML, RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, PARK2, WRN, Sp100, IkB-alpha, Androgen receptor (AR), GLUT1/4, CaMK, DNMT3B, TDG, HIF1A, CHD3, EXOSC9, RAD51, and viral targets such as CMV-IE1/2, EBV-BZLF1, and HPV/BPV-E1.

Molekulargewicht: 11637

Gen-ID: 6612

NCBI Accession: NP_008867

UniProt: P55854