Aktivieren Sie Javascript in Ihrem Browser, um unsere Webseite optimal zu nutzen.

PUMA Antikörper (AA 119-154)

Name: PUMA Antikörper (AA 119-154)
SKU: ABIN388112
Price: 445.5 EUR
Availability: InStock

BBC3 Reaktivität: Human WB, FACS, IHC (p), IF Wirt: Kaninchen Polyclonal RB01353 unconjugated

Western blot analysis of anti-Puma BH3 domain Pab (ABIN388112 and ABIN2846254) in HL-60 cell lysate. (PUMA Antikörper (AA 119-154))

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody (ABIN388112 and ABIN2846254) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (PUMA Antikörper (AA 119-154))

IHC (p)

Confocal immunofluorescent analysis of Puma BH3 Domain Antibody (ABIN388112 and ABIN2846254) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (PUMA Antikörper (AA 119-154))

Puma BH3 Domain Antibody (ABIN388112 and ABIN2846254) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). (PUMA Antikörper (AA 119-154))

FACS

4 Abbildungen

(1 reference)

Produktnummer ABIN388112

Datenblatt (PDF)

Target Alle PUMA (BBC3) Antikörper anzeigen

PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
Bindungsspezifität
16

13

9

7

7

6

6

6

5

5

4

2

1

1

1

1

1

1

1

1
AA 119-154
Reaktivität
91

40

38

16
Human
Wirt
88

6

1
Kaninchen
Passende Sekundärantikörper
Klonalität
80

15
Polyklonal
Konjugat
47

6

5

5

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Dieser PUMA Antikörper ist unkonjugiert
Applikation
76

34

29

20

15

14

13

11

9

7

5

3

2

2
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Homologie

M, Rat

Aufreinigung

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This Puma BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from human Puma BH3 Domain.

Klon

RB01353

Isotyp

Ig Fraction

Top Product

Discover our top product BBC3 Primärantikörper
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody
BBC3 Reaktivität: Human WB, ICC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 131-180) antibody
BBC3 Reaktivität: Human, Ratte, Schwein WB, ELISA, IP, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 119-154) antibody
BBC3 Reaktivität: Human WB, ELISA, IHC, FACS, IF Wirt: Kaninchen Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibody
BBC3 Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (PE)
BBC3 Reaktivität: Human WB, ICC, IF Wirt: Kaninchen Polyclonal PE

anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (PerCP)
BBC3 Reaktivität: Human WB, ICC, IF Wirt: Kaninchen Polyclonal PerCP

anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibody (Biotin)
BBC3 Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal Biotin

anti-BCL2 Binding Component 3 (BBC3) (BH3 Domain), (C-Term) antibody
BBC3 Reaktivität: Human, Maus WB, FACS, IHC (p), IF, EIA Wirt: Kaninchen Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 109-137) antibody
BBC3 Reaktivität: Human WB, IHC (p) Wirt: Kaninchen Polyclonal RB33714 unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 98-152) antibody
BBC3 Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 5D10 unconjugated

Applikationshinweise

IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit

6 months
Vesela, Matalova: "Detection of Bim and Puma in mouse hair follicles using immunofluorescence and TUNEL assay double staining." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, pp. 1-7, (2015) (PubMed).
Target

PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Andere Bezeichnung

Puma (BBC3 Produkte)

Synonyme

BBC3 antikoerper, Puma antikoerper, JFY-1 antikoerper, JFY1 antikoerper, PUMA antikoerper, PUMA/JFY1 antikoerper, BCL2 binding component 3 antikoerper, Bcl-2 binding component 3 antikoerper, BBC3 antikoerper, Bbc3 antikoerper, bbc3 antikoerper

Hintergrund

PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Molekulargewicht

20532

Gen-ID

27113

NCBI Accession

NP_001120712, NP_001120713, NP_001120714, NP_055232

UniProt

Q9BXH1

Pathways

p53 Signalweg, Positive Regulation of Endopeptidase Activity